Chicken Interferon β,IFN-β/IFNB ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

鸡β干扰素(IFN-β/IFNB)酶联免疫试剂盒（CSB-E11237Ch）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的IFN-β/IFNB含量。IFN-β/IFNB即β-干扰素，是机体受病毒感染后产生的一类细胞因子。它能与细胞表面受体结合，激活下游信号通路，诱导抗病毒蛋白产生，抑制病毒复制和调节免疫反应，在抗病毒、抗肿瘤及免疫相关疾病研究中受关注。试剂盒检测范围为2.5 pg/mL-200 pg/mL，适用于科研场景中禽类免疫机制研究、疫苗效力评价或抗病毒药物开发等方向；可应用于评估病毒感染模型、免疫刺激实验或比较不同组织微环境中IFN-β的动态表达水平本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

IFNB ELISA Kit; IFN2 ELISA Kit; Interferon type B ELISA Kit; IFN-beta ELISA Kit
缩写：

IFN-β/IFNB
Uniprot No.：

Q90873
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2.5 pg/mL-200 pg/mL
灵敏度：

3.125 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IFN-β in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	99
	Range %	95-103
1:2	Average %	94
	Range %	88-98
1:4	Average %	102
	Range %	98-106
1:8	Average %	89
	Range %	84-96

回收率：

The recovery of chicken IFN-β spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	88-94
EDTA plasma (n=4)	88	82-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Has antiviral activities.
基因功能参考文献：
1. Fpv012 inhibits induction of IFNB. PMID: 23427153
2. RNA interference-mediated knockdown of ChMda5 showed that ChMda5 plays an important role in the IFN response of chicken cells to dsRNA PMID: 21444763
亚细胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
数据库链接：

KEGG: gga:554219

STRING: 9031.ENSGALP00000038687

UniGene: Gga.22865