Chicken Immunoglobulin of Yolk,IgY ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO鸡卵黄免疫球蛋白（IgY）酶联免疫检测试剂盒（货号：CSB-E11635Ch），采用夹心法原理实现高灵敏度定量检测，适用于血清、血浆及蛋黄样本中IgY的精准分析。IgY作为禽类特有的免疫球蛋白，在蛋黄中含量丰富，其功能类似于哺乳动物的IgG，是研究禽类免疫应答机制、抗体开发及疫苗评价的重要靶标。本试剂盒检测范围覆盖0.078 ng/mL至5 ng/mL，灵敏度满足微量样本检测需求，操作流程中通过预包被特异性捕获抗体与标记检测抗体的协同作用，确保检测结果的高特异性与稳定性。该产品可广泛应用于家禽免疫学研究、疫苗免疫效果评估、抗体生产工艺优化及生物制品质量控制等科研场景，为禽类相关疾病模型的免疫指标分析提供可靠工具，实验全程需严格按照说明书操作以确保数据准确性。
缩写：

IgY
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, egg yolk
检测范围：

0.078 ng/mL-5 ng/mL
灵敏度：

0.02 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken IgY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:500000	Average %	89
	Range %	81-100
1:1000000	Average %	96
	Range %	87-103
1:2000000	Average %	97
	Range %	92-104
1:4000000	Average %	92
	Range %	86-98

回收率：

The recovery of chicken IgY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-105
EDTA plasma (n=4)	97	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

最新研究进展：

IgY，即鸟类IgY免疫球蛋白，是由鸟类产生的一种特殊类型的免疫球蛋白。最近的研究表明，IgY可以在多个领域中发挥重要作用，包括食品和医药工业。例如，IgY被用作生产抗体、免疫增强剂和生物传感器的材料。此外，IgY还被用作一种潜在的治疗肠道疾病的药物。