Chicken Glutathione Peroxidase 8 (GPX-8) ELISA Kit

产品详情

产品描述：

鸡谷胱甘肽过氧化物酶8(GPX-8)酶联免疫试剂盒（CSB-E14942C）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GPX-8含量。鸡谷胱甘肽过氧化物酶8（GPX-8）是一种位于内质网的非特异性抗氧化酶，参与清除活性氧，保护细胞膜免受氧化损伤。其基因突变与肿瘤发生密切相关，在多种肿瘤组织中异常表达，影响肿瘤免疫微环境和侵袭转移。试剂盒检测范围为31.25 mU/mL-2000 mU/mL，该产品可广泛应用于家禽氧化应激机制研究、营养代谢评估、毒理学实验及疾病模型建立等科研领域，例如评估饲料抗氧化剂效果、监测环境毒素暴露对家禽抗氧化系统的影响，或探究传染性疾病进程中GPX - 8的动态变化规律，为禽类健康管理和基础医学研究提供可靠的技术支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

GPX-8
Uniprot No.：

R4GGB2
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 mU/mL-2000 mU/mL
灵敏度：

7.8 mU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken GPX-8 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	98
	Range %	94-103
1:200	Average %	86
	Range %	83-90
1:400	Average %	104
	Range %	100-108
1:800	Average %	89
	Range %	85-93

回收率：

The recovery of chicken GPX-8 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	107	103-110
EDTA plasma (n=4)	84	80-88

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.