Chicken Ghrelin ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本产品CSB-E14230C为鸡饥饿激素（Ghrelin）定量检测试剂盒，采用竞争性酶联免疫吸附法（ELISA）原理，适用于检测血清、血浆及组织匀浆样本中Ghrelin的浓度水平。Ghrelin是一种由胃肠道分泌的多肽激素，在家禽生理调节中参与摄食调控、能量代谢平衡及生长激素释放等过程，是研究禽类代谢与生长机制的重要生物标志物。试剂盒检测范围为25 pg/mL至400 pg/mL，最低检测灵敏度为25 pg/mL，样本需求量为50 μL，可覆盖常规科研场景下的浓度检测需求。该试剂盒通过预先包被的抗体与样本中Ghrelin竞争结合酶标抗原，经显色反应后通过标准曲线进行定量分析，操作流程包含样本预处理、孵育、洗涤及读数步骤，适用于家禽生理学研究、营养代谢实验、生长调控机制探索等科研领域，如评估饲料成分对Ghrelin分泌的影响或分析激素水平与生长性能的关联性，为禽类生物学研究提供可靠工具。
缩写：

Ghrelin
种属：

Gallus gallus (Chicken)
样本类型：

serum, plasma, tissue homogenates
检测范围：

25 pg/mL-400 pg/mL
灵敏度：

25 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of chicken ghrelin in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	87-96
1:2	Average %	90
	Range %	86-95
1:4	Average %	95
	Range %	90-100
1:8	Average %	97
	Range %	92-103

回收率：

The recovery of chicken ghrelin spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-98
EDTA plasma (n=4)	93	88-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.