Canine kidney injury molecule 1(Kim-1) ELISA Kit

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

To assess the linearity of the assay, samples were spiked with high concentrations of canine Kim-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

Sample

Serum(n=4)

1:1

Average %

91

Range %

86-95

1:2

Average %

102

Range %

97-107

1:4

Average %

91

Range %

85-97

1:8

Average %

97

Range %

91-103

The recovery of canine Kim-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type

Average % Recovery

Range

Serum (n=5)

87

80-93

EDTA plasma (n=4)

90

83-96

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

Corrected

20

2.449

2.563

2.506

2.348

10

1.595

1.538

1.567

1.409

5

1.001

1.052

1.027

0.869

2.5

0.687

0.648

0.668

0.510

1.25

0.463

0.473

0.468

0.310

0.625

0.306

0.314

0.310

0.152

0.312

0.221

0.232

0.227

0.069

0

0.154

0.161

0.158