Canine follicle-stimulating hormone,FSH ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬促卵泡素（FSH）是一种重要的糖蛋白激素，在犬类生殖系统中参与调控卵泡发育、精子生成以及性激素分泌等生理过程。本试剂盒（货号CSB-E06868c）采用竞争法原理，可定量检测犬血清、血浆及组织匀浆样本中的FSH水平，检测灵敏度达0.35 mIU/mL，线性检测范围为0.35-140 mIU/mL，适用于低浓度样本的精准分析。该试剂盒通过预包被特异性抗体与样本中的FSH及标记物竞争结合，结合酶标检测系统实现目标物的定量测定，操作流程标准化且重复性良好。科研人员可将其应用于犬类生殖生理学研究、繁殖障碍机制探索、生殖相关药物或营养干预效果评估等领域，例如通过检测发情周期、卵巢功能异常或睾丸发育相关样本中的FSH动态变化，为犬科动物繁殖生物学研究提供可靠的数据支持。试剂盒包含完整实验组分及操作指南，需自备酶标仪等基础实验设备，严格遵循说明书进行操作可获得稳定检测结果。
缩写：

FSH
Uniprot No.：

E2RJZ8
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.35 mIU/mL-140 mIU/mL
灵敏度：

0.35 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-106
1:2	Average %	95
	Range %	90-105
1:4	Average %	98
	Range %	93-104
1:8	Average %	92
	Range %	85-99

回收率：

The recovery of canine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	91-99
EDTA plasma (n=4)	96	92-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.