Canine connective tissue growth factor,CTGF ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬结缔组织生长因子（CTGF）酶联免疫检测试剂盒（货号：CSB-E13386c），主要用于科研领域中对犬血清、血浆或组织匀浆样本中CTGF蛋白的定量分析。CTGF是一种参与细胞外基质重塑和纤维化过程的关键调控因子，在组织修复、慢性炎症及器官纤维化等病理生理过程中发挥重要作用。CUSABIO试剂盒采用双抗体夹心法原理，通过特异性抗体对靶标进行高灵敏度捕获与检测，其线性检测范围为62.5-4000 pg/mL，可精准检测样本中CTGF的浓度变化。该试剂盒适用于犬类纤维化疾病模型研究、器官损伤修复机制探索以及相关代谢性疾病的分子机制分析等科研场景，为研究者提供稳定可靠的实验工具。试剂盒操作便捷，配套标准品及实验所需试剂组分齐全，需自备酶标仪等基础实验设备。实验过程需严格遵守说明书操作规范以确保数据准确性。
缩写：

CTGF
Uniprot No.：

J9NTX8
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine CTGF in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	91
	Range %	86-96
1:2	Average %	97
	Range %	93-101
1:4	Average %	85
	Range %	81-89
1:8	Average %	92
	Range %	85-97

回收率：

The recovery of canine CTGF spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	108	103-111

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.