Canine cardiac isoform of Tropnin T,cTnT ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬心肌特异性肌钙蛋白T(cTnT)酶联免疫试剂盒（CSB-E14377c）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的cTnT含量。cTnT即心肌肌钙蛋白T，在心肌损伤检测方面意义重大。正常时血液中含量极低，心肌受损时释放入血。研究机制主要通过检测其在血液中的含量变化，来评估心肌损伤的程度和范围，是诊断急性心肌梗死等疾病的关键指标。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，在心血管疾病机制研究、心脏毒性评估及动物模型中具有重要科研价值；适用于探索犬类心脏疾病模型、药物干预对心肌的影响或心肌相关分子机制研究。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

cTnT
Uniprot No.：

J9P6P7
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

3.9 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine cTnT in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	85-105
1:2	Average %	95
	Range %	85-103
1:4	Average %	94
	Range %	82-100
1:8	Average %	95
	Range %	85-105

回收率：

The recovery of canine cTnT spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	82-104
EDTA plasma (n=4)	92	85-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.