Canine acetylcholine receptor antibody,AChRab ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬乙酰胆碱受体抗体(AChRab)酶联免疫试剂盒（CSB-E08375c）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的AChR Ab含量。AChR Ab即乙酰胆碱受体抗体，它与重症肌无力等疾病密切相关。其背景在于机体免疫系统异常产生该抗体。研究机制主要是它会与神经肌肉接头处的乙酰胆碱受体结合，干扰神经冲动传导，影响肌肉正常收缩，是相关疾病发病的重要因素。试剂盒检测范围为3.75 ng/mL-240 ng/mL，适用于基础科研中探索AChRab在神经功能障碍模型中的表达变化，或用于药物开发过程中评估实验性治疗对抗体水平的调控作用，同时也可作为犬类自身免疫疾病研究中的生物标志物检测方案，为动物模型的建立及病理机制解析提供数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

AChR Ab
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma
检测范围：

3.75 ng/mL-240 ng/mL
灵敏度：

0.94 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine AChRab in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	89-102
1:2	Average %	95
	Range %	84-100
1:4	Average %	96
	Range %	90-103
1:8	Average %	94
	Range %	82-101

回收率：

The recovery of canine AChRab spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-101
EDTA plasma (n=4)	92	87-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.