Canine Progesterone,PROG ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬孕激素/孕酮（PROG）酶联免疫试剂盒（货号：CSB-E07285c）专为科研领域设计，可精准检测犬血清、血浆及组织匀浆样本中的PROG浓度。孕酮作为哺乳动物生殖系统的重要激素，在调节雌性犬类发情周期、胚胎着床及妊娠维持中发挥关键作用，其动态监测对繁殖生物学研究具有重要意义。CUSABIO试剂盒采用竞争法酶联免疫吸附技术，通过预包被特异性抗体与样本中PROG及酶标记物竞争结合的原理，实现0.15-70 ng/ml宽线性范围内的精确定量，灵敏度达0.15 ng/ml。试剂盒包含预装96孔板、标准品、检测抗体及显色底物等完整组分，实验全程仅需约3小时，支持手动或自动化仪器操作。适用于动物繁殖生理研究、生殖内分泌机制探索、药物对卵巢功能影响评估等场景，尤其可为犬类人工授精时机判断、妊娠监测及生殖疾病模型构建提供可靠数据支持。样本兼容性强，组织匀浆需经离心预处理后检测。
缩写：

PROG
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates.
检测范围：

0.15 ng/ml-70 ng/ml.
灵敏度：

0.2 ng/ml.
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine PROG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.

	Sample	Serum(n=4)
1:1	Average %	96
	Range %	92-104
1:2	Average %	94
	Range %	91-99
1:4	Average %	102
	Range %	98-105
1:8	Average %	89
	Range %	82-94

回收率：

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml

OD1

OD2

Average

2.284

2.275

2.280

0.15

1.429

1.589

1.509

0.843

0.874

0.859

0.566

0.585

0.576

0.362

0.353

0.358

0.201

0.202