Canine Matrix metalloproteinase 2/Gelatinase A,MMP-2 ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬基质金属蛋白酶2/明胶酶A（MMP-2/Gelatinase A）酶联免疫试剂盒（货号：CSB-E14056c）是一款定量检测犬血清、血浆及组织匀浆样本中MMP-2浓度的科研工具。MMP-2作为基质金属蛋白酶家族成员，主要参与细胞外基质降解，在组织重塑、炎症调控及肿瘤微环境调节等生理病理过程中发挥重要作用。本试剂盒基于双抗体夹心法原理，通过预包被特异性抗体实现高灵敏度检测，线性范围为4.69 ng/mL至300 ng/mL，可覆盖常见样本中MMP-2的表达水平。适用于犬类疾病模型研究中MMP-2动态变化的监测，如肿瘤侵袭转移机制分析、伤口愈合评估或心血管疾病相关基质重塑研究。试剂盒兼容多种生物样本类型，为科研工作者提供稳定可靠的检测方案，助力探索MMP-2在疾病发生发展中的分子机制。
缩写：

MMP2/Gelatinase A
Uniprot No.：

F1PMK7
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

4.69 ng/mL-300 ng/mL
灵敏度：

1.17 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	94-106
1:2	Average %	85
	Range %	80-95
1:4	Average %	95
	Range %	92-100
1:8	Average %	93
	Range %	86-98

回收率：

The recovery of canine MMP-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	90-101
EDTA plasma (n=4)	92	87-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.