Canine Matrix metalloproteinase 13,MMP-13 ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心法原理定量检测犬基质金属蛋白酶13（MMP-13），该蛋白属于基质金属蛋白酶家族，在细胞外基质重塑、炎症调控及软骨代谢等生理病理过程中发挥重要作用。试剂盒适用于犬血清、血浆、细胞培养上清及组织匀浆样本分析，检测灵敏度达0.156 ng/mL，线性检测范围为0.156-10 ng/mL，可精确捕捉微量目标蛋白浓度变化。实验体系通过预包被特异性捕获抗体与生物素标记检测抗体的协同作用，结合链霉亲和素-HRP显色系统实现目标蛋白定量分析，适用于科研领域对犬类骨关节疾病模型、软骨退行性病变机制以及抗炎药物研发等研究方向的样本检测需求。试剂盒操作标准化，提供完整实验组分包含预包被板、标准品、检测抗体及终止液等核心试剂，满足实验室对犬MMP-13蛋白表达水平的高通量检测及动态监测要求。
缩写：

MMP-13
Uniprot No.：

F1PVS9
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

0.156 ng/mL-10 ng/mL
灵敏度：

0.039 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-13 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	88-99
1:2	Average %	99
	Range %	95-106
1:4	Average %	87
	Range %	84-92
1:8	Average %	91
	Range %	85-98

回收率：

The recovery of canine MMP-13 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	87-95
EDTA plasma (n=4)	88	85-92

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.