Canine Matrix metalloproteinase 1,MMP-1 ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO犬基质金属蛋白酶1（MMP-1）酶联免疫检测试剂盒（货号：CSB-E14057c），采用双抗体夹心法定量分析犬血清、血浆或组织匀浆样本中的MMP-1浓度。MMP-1作为基质金属蛋白酶家族成员，在细胞外基质降解、组织重塑及炎症反应中起关键作用，其表达水平与多种病理过程相关。本试剂盒检测灵敏度可达0.18 ng/mL，线性范围为0.18-12 ng/mL，适用于科研场景中探究犬类疾病模型（如关节炎、皮肤损伤修复或肿瘤微环境）中MMP-1的动态变化。试剂盒包含预包被特异性抗体的96孔板、标准品、检测抗体及显色系统，操作流程约需4.5小时完成，支持批量样本的高通量分析。通过优化抗体配对和反应体系，该产品对复杂生物样本（如富含蛋白的组织匀浆）具有良好兼容性，实验结果稳定可靠，可为分子机制研究、药物开发或生物标志物筛选提供定量数据支持，满足科研工作者对犬类MMP-1活性监测的需求。
缩写：

MMP-1
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.18 ng/mL-12 ng/mL
灵敏度：

0.04 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cancer
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine MMP-1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	93
	Range %	85-102
1:2	Average %	89
	Range %	85-99
1:4	Average %	94
	Range %	85-97
1:8	Average %	90
	Range %	83-98

回收率：

The recovery of canine MMP-1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	82-98
EDTA plasma (n=4)	97	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.