Canine Luteinizing Hormone,LH ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬黄体生成素(LH)酶联免疫试剂盒（CSB-E06790c）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的LH含量。LH即促黄体生成素，是腺垂体分泌的糖蛋白类促性腺激素。它在生殖系统中至关重要，参与调节女性月经周期和排卵、男性睾酮合成。研究机制主要围绕其与受体结合，激活下游信号通路，影响性腺功能和生殖内分泌稳态。试剂盒检测范围为0.75 mIU/mL-60 mIU/mL，适用于生殖内分泌研究、繁殖障碍机制探索、绝育手术前后激素水平追踪以及繁育管理中的排卵期监测等科研场景，为动物生殖生物学、兽医学基础研究及宠物繁育技术开发提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

LHBLutropin subunit beta ELISA Kit; Luteinizing hormone subunit beta ELISA Kit; LH-B ELISA Kit; LSH-B ELISA Kit; LSH-beta ELISA Kit; Lutropin beta chain ELISA Kit; Fragment ELISA Kit
缩写：

LH
Uniprot No.：

P18842
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.75 mIU/mL-60 mIU/mL
灵敏度：

0.94 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine LH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	94
	Range %	90-98
1:2	Average %	96
	Range %	92-99
1:4	Average %	101
	Range %	98-105
1:8	Average %	83
	Range %	80-87

回收率：

The recovery of canine LH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	86	84-92
EDTA plasma (n=4)	98	92-104

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Promotes spermatogenesis and ovulation by stimulating the testes and ovaries to synthesize steroids.
亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

KEGG: cfa:403959

STRING: 9615.ENSCAFP00000005719

UniGene: Cfa.3817