Canine Interferon alpha-1/2 ELISA kit

Canine Interferon alpha-1/2 ELISA kit

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中文名称：

犬α-1/2干扰素(IFN-α1/2)酶联免疫试剂盒
货号：

CSB-E09849c
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

犬α-1/2干扰素(IFN-α1/2)酶联免疫试剂盒（CSB-E09849c）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的IFN-A1/2含量。IFN-A1/2属干扰素家族，在机体免疫防御中起关键作用。研究发现其能激活细胞内信号通路，诱导抗病毒蛋白表达以抵御病毒感染。还参与调节免疫细胞活性和功能，在对抗肿瘤及自身免疫病中展现潜在价值，是研究热点。试剂盒检测范围为12.5 pg/mL-800 pg/mL，适用于科研场景中犬类I型干扰素的功能研究；其动态水平变化常被用于评估病毒感染模型、疫苗免疫原性实验或免疫调节相关机制研究；细胞培养上清液可分析体外刺激后免疫细胞分泌情况，组织匀浆则适用于疾病模型中局部干扰素水平的定量分析；为动物免疫学、病毒学及炎症机制研究提供可靠工具本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Interferon alpha-1/2 ELISA Kit; IFN-alpha-1/2 ELISA Kit
缩写：

IFN-α1/2
Uniprot No.：

P81255
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.12 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine IFN-α1/2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	86-99
1:2	Average %	100
	Range %	94-109
1:4	Average %	92
	Range %	89-96
1:8	Average %	87
	Range %	83-96

回收率：

The recovery of canine IFN-α1/2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	85-96
EDTA plasma (n=4)	94	88-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.815	2.824	2.820	2.726
400	2.417	2.425	2.421	2.327
200	1.834	1.863	1.849	1.755
100	1.042	1.112	1.077	0.983
50	0.518	0.563	0.541	0.447
25	0.248	0.269	0.259	0.165
12.5	0.126	0.131	0.129	0.035
0	0.091	0.096	0.094

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Produced by macrophages, IFN-alpha have antiviral activities. Interferon stimulates the production of two enzymes: a protein kinase and an oligoadenylate synthetase.
亚细胞定位：

Secreted.
蛋白家族：

Alpha/beta interferon family
数据库链接：

KEGG: cfa:100685213

UniGene: Cfa.4550

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.