Canine Fibroblast Growth Factor 23 (FGF23) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

犬成纤维细胞生长因子23(FGF23)酶联免疫试剂盒（CSB-E17661c）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF23含量。FGF23（成纤维细胞生长因子23）是一种由骨骼和肾脏分泌的激素，主要参与调节磷酸盐和钙的代谢。其研究背景涉及慢性肾病、低磷血症等疾病。FGF23通过与Klotho蛋白结合，调节肾小管对磷酸盐的重吸收，影响维生素D代谢和甲状旁腺激素的分泌。研究机制主要围绕其与骨骼、肾脏和神经系统之间的相互作用，以及在不同疾病状态下的功能变化。试剂盒检测范围为3.12 pg/mL-200 pg/mL，适用于科研领域中对犬类慢性肾病、钙磷代谢紊乱及相关内分泌疾病的机制探索，亦可用于动物模型中骨矿化异常或激素调控路径的基础研究本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

ADHR, HPDR2, HYPF, PHPTC, tumor-derived hypophosphatemia inducing factor
缩写：

FGF23
种属：

Canis lupus familiaris (Dog) (Canis familiaris)
样本类型：

serum, plasma, tissue homogenates
检测范围：

3.12 pg/mL-200 pg/mL
灵敏度：

0.78 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of canine FGF23 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	98
	Range %	95-101
1:2	Average %	87
	Range %	83-91
1:4	Average %	98
	Range %	94-102
1:8	Average %	84
	Range %	81-86

回收率：

The recovery of canine FGF23 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	85-95
EDTA plasma (n=4)	93	90-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.