Bovine pulmonary surfactant-associated protein C,SP-C ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛肺表面活性物质相关蛋白C(SP-C)酶联免疫试剂盒（CSB-E13835B）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPC含量。SFTPC编码的蛋白质是肺表面活性物质的组成部分，对维持肺部功能至关重要。其突变与多种肺部疾病相关，如间质性肺疾病。研究机制主要围绕突变如何影响蛋白结构和功能、干扰表面活性物质稳态，进而引发肺部病变展开。试剂盒检测范围为12.5 ng/mL-800 ng/mL，本产品适用于科研领域探索牛肺部疾病的分子机制、评估肺损伤模型的生物标志物变化，或用于农业研究中监测反刍动物肺部健康状态。为畜牧学、兽医学及基础医学研究提供高效检测工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

SFTPC ELISA Kit; SFTP2 ELISA Kit; Pulmonary surfactant-associated protein C ELISA Kit; SP-C ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Val) ELISA Kit
缩写：

SFTPC
Uniprot No.：

P15783
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 ng/mL-800 ng/mL
灵敏度：

3.12 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-C in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	96
	Range %	93-99
1:2	Average %	110
	Range %	107-113
1:4	Average %	95
	Range %	91-99
1:8	Average %	90
	Range %	87-93

回收率：

The recovery of bovine SP-C spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-96
EDTA plasma (n=4)	106	101-110

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Pulmonary surfactant associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces.
基因功能参考文献：
1. The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases. PMID: 20409474
亚细胞定位：

Secreted, extracellular space, surface film.
数据库链接：

KEGG: bta:282071

STRING: 9913.ENSBTAP00000016672

UniGene: Bt.8126