Bovine pulmonary surfactant-associated protein B,SP-B ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛肺表面活性物质相关蛋白B(SP-B)酶联免疫试剂盒（CSB-E13834B）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的SFTPB含量。试剂盒检测范围为9.37 ng/mL-600 ng/mL，适用于研究SP - B在肺部生理功能、病理机制（如急性肺损伤或发育异常）中的表达调控，也可用于评估动物模型中药物干预或基因编辑对SP - B水平的影响。其兼容多种样本类型的特点，为科研人员在基础研究和转化医学领域探索呼吸系统相关分子机制提供了高效、可靠的分析工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

SFTPB ELISA Kit; SFTP3 ELISA Kit; Pulmonary surfactant-associated protein B ELISA Kit; SP-B ELISA Kit; 6 kDa protein ELISA Kit; Pulmonary surfactant-associated proteolipid SPL(Phe) ELISA Kit
缩写：

SFTPB
Uniprot No.：

P15781
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

9.37 ng/mL-600 ng/mL
灵敏度：

2.34 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SP-B in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	97
	Range %	92-103
1:2	Average %	91
	Range %	85-97
1:4	Average %	90
	Range %	88-94
1:8	Average %	100
	Range %	96-105

回收率：

The recovery of bovine SP-B spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	87-93
EDTA plasma (n=4)	106	103-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Pulmonary surfactant-associated proteins promote alveolar stability by lowering the surface tension at the air-liquid interface in the peripheral air spaces. SP-B increases the collapse pressure of palmitic acid to nearly 70 millinewtons per meter.
基因功能参考文献：
1. Measurements of the depth of SP-B(CTERM) indicated the peptide center positions ~8A more deeply than the phosphate headgroups, a topology that may allow the peptide to promote functional lipid structures without causing micellization upon compression. PMID: 22252270
2. results suggest the hydrophobic surfactant protein, SP-B, protects alveolar surfactant phospholiopids from hydrolysis mediated by multiple sPLA(2) in both vesicles (alveolar subphase) and monomolecular films (air-liquid interface) PMID: 22037357
3. The hydrophobic surfactant proteins SP-B and SP-C induce formation of bicontinuous inverse cubic phases. PMID: 20409474
4. Reactive oxygen species inactivation of lipid extract surfactant B (SP-B) biophysical activity appears to involve modification of a maximum of three amino acids: Trp9, Met29, and Met65. PMID: 17425286
亚细胞定位：

Secreted, extracellular space, surface film.
数据库链接：

KEGG: bta:507398

STRING: 9913.ENSBTAP00000042549

UniGene: Bt.42696