Bovine neuropeptide Y (NPY) ELISA kit

Bovine neuropeptide Y (NPY) ELISA kit

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中文名称：

牛神经肽Y(NPY)酶联免疫试剂盒
货号：

CSB-E08171b
规格：

96T/48T
价格：

￥4800/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

牛神经肽Y(NPY)酶联免疫试剂盒（CSB-E08171b）为双抗夹心法ELISA试剂盒，定量检测血清、血浆样本中的NPY含量。NPY即神经肽Y，是一种广泛分布于中枢和外周神经系统的肽类神经递质。它在调节摄食行为、能量代谢、心血管活动等方面发挥重要作用。研究机制上，主要通过与相应受体结合，激活细胞内信号转导途径，进而产生一系列生理效应。试剂盒检测范围为0.312 ng/mL-20 ng/mL，该产品可用于科研领域探索NPY在牛的能量代谢调控、应激生理响应、神经内分泌网络中的作用机制，例如在饲喂干预、环境压力模型或神经功能研究中动态监测NPY的表达水平变化，为畜牧学、动物生理学及比较医学研究提供可靠的工具支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

NPY ELISA Kit; Pro-neuropeptide Y [Cleaved into: Neuropeptide Y ELISA Kit; Neuropeptide tyrosine ELISA Kit; NPY); C-flanking peptide of NPY ELISA Kit; CPON)] ELISA Kit
缩写：

NPY
Uniprot No.：

Q6RUW3
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

0.312 ng/mL-20 ng/mL
灵敏度：

0.078 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine NPY in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	87-93
1:2	Average %	102
	Range %	98-105
1:4	Average %	90
	Range %	86-96
1:8	Average %	91
	Range %	88-97

回收率：

The recovery of bovine NPY spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	86-94
EDTA plasma (n=4)	91	87-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average	Corrected
20	2.604	2.490	2.547	2.444
10	2.113	2.221	2.167	2.064
5	1.657	1.711	1.684	1.581
2.5	1.114	1.056	1.085	0.982
1.25	0.712	0.687	0.700	0.597
0.625	0.413	0.428	0.421	0.318
0.312	0.278	0.260	0.269	0.166
0	0.105	0.101	0.103

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

NPY is implicated in the control of feeding and in secretion of gonadotrophin-release hormone.
基因功能参考文献：
1. Hormonal gene expression involved in residual feed intake in dairy cows may be related to the molecular regulation of the leptin-NPY and insulin signaling pathways. PMID: 26231801
2. Data indicate that increased body weight gain during juvenile development accelerates sexual maturation in heifers, coincident with reciprocal changes in circulating concentrations of leptin and hypothalamic neuropeptide Y (NPY) release. PMID: 25326602
3. There is a strong association between putative favorable allelic variants (SNP) of neuropeptide Y, leptin, and IGF-1 genes, and residual feed intake, when animals were grazing on a high-quality, high-availability pasture. PMID: 23881687
4. The NPY SNP (NPY1) was associated with the prevalence of the animal being in calf 100 d after calving and 305-d milk yield in the first lactation. PMID: 21700051
5. NPY tended to increase serum growth hormone, which appeared to be a consequence of increased pulse amplitude. Infusion of NPY also increased CSF growth hormone-releasing hormone. PMID: 19632078
6. The effect of single nucleotide polymorphisms in 6 genes and their associations with production factors in beef cattle are reported. PMID: 17785604
7. Data show that PKA and PKC pathways are involved in the differential regulation of production of the neuropeptides (Met)enkephalin, galanin, somatostatin, NPY, and VIP. PMID: 18619673
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亚细胞定位：

Secreted. Cytoplasmic vesicle, secretory vesicle, neuronal dense core vesicle.
蛋白家族：

NPY family
数据库链接：

KEGG: bta:504216

STRING: 9913.ENSBTAP00000005903

UniGene: Bt.33726

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.