Bovine follicle-stimulating hormone(FSH)ELISA Kit

Bovine follicle-stimulating hormone(FSH)ELISA Kit

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中文名称：

牛促卵泡素(FSH)酶联免疫试剂盒
货号：

CSB-E15856B
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO牛促卵泡素（FSH）酶联免疫检测试剂盒（货号：CSB-E15856B），采用竞争法定量原理，可精准检测血清、血浆及组织匀浆样本中的FSH水平，覆盖2-800 mIU/mL的宽线性范围，满足科研场景中不同浓度样本的检测需求。FSH作为垂体分泌的糖蛋白激素，在牛生殖调控中发挥核心作用，通过促进卵泡发育、配子成熟及性腺功能调节参与繁殖生理过程。该试剂盒基于高特异性抗体与样本中FSH竞争结合标记物的原理，可有效区分目标分子，尤其适用于复杂生物基质中低丰度FSH的定量分析，为繁殖生物学研究、生殖疾病机制探索、促排卵药物效果评估等提供可靠工具。其兼容多种样本类型的特点，支持研究者从血液循环系统到性腺组织微环境的系统性分析，例如卵巢卵泡发育动态监测、睾丸功能评估或垂体-性腺轴调控机制研究，为畜牧繁殖优化、生殖内分泌学基础研究及兽药开发等科研领域提供精准数据支持。
别名：

FSHB ELISA Kit; Follitropin subunit beta ELISA Kit; Follicle-stimulating hormone beta subunit ELISA Kit; FSH-B ELISA Kit; FSH-beta ELISA Kit; Follitropin beta chain ELISA Kit
缩写：

FSH
Uniprot No.：

P04837
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

2 mIU/mL-800 mIU/mL
灵敏度：

2 mIU/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FSH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	91-105
1:2	Average %	93
	Range %	89-100
1:4	Average %	95
	Range %	89-100
1:8	Average %	97
	Range %	92-107

回收率：

The recovery of bovine FSH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	89-96
EDTA plasma (n=4)	94	90-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

mIU/ml	OD1	OD2	Average
800	0.448	0.439	0.444
150	0.821	0.824	0.823
25	1.354	1.329	1.342
7	1.711	1.705	1.708
2	2.019	2.011	2.015
0	2.555	2.566	2.561

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

Rumen-protected glucose stimulates the secretion of reproductive hormones and the mTOR/AKT signaling pathway in the ovaries of early postpartum Y Wang,researchsquare,2022
Bone Marrow-Derived Mesenchymal Stem Cells Reverse Radiotherapy-Induced Premature Ovarian Failure: Emphasis on Signal Integration of TGF-β, Wnt/β-Catenin and Hippo Pathways MO El-Derany,Stem Cell Reviews and Reports,2021
Regulation of AMH, AMHR-II, and BMPs (2,6) Genes of Bovine Granulosa Cells Treated with Exogenous FSH and Their Association with Protein Hormones Umer S, et al,Genes,2019
Fixed-time induction of ovulation in camels superovulated by different eCG modalities during the transition period in Egypt : Superovulation in camels during the transition period. Marwa Ahmed Khalifa.et al,Tropical Animal Health and Production,2016

功能：

Together with the alpha chain CGA constitutes follitropin, the follicle-stimulating hormone, and provides its biological specificity to the hormone heterodimer. Binds FSHR, a G protein-coupled receptor, on target cells to activate downstream signaling pathways. Follitropin is involved in follicle development and spermatogenesis in reproductive organs.
基因功能参考文献：
1. Study describes generation and characterization of a new Fshb-iCre deleter strain using 4.7 kb of ovine Fshb promoter sequences driving iCre expression exclusively in the gonadotrope lineage with mice developing normally, and fertile. PMID: 26472536
2. the novel cis-regulatory element generated by the c.-1539_-1538delGGinsTTAACT mutations might play an important role in repressing FSHB transcription, possibly leading to reduced fertility PMID: 26478576
3. A 200-base region in the bovine FSHbeta 3' untranslated region downregulates reporter expression at the translational level via interactions between AREs in both the 3'UTR and transfactors. PMID: 15189830
4. The shift in FSH isoforms toward a greater abundance of the less acidic isoforms appears to be a key component in determining the capability for producing a preovulatory rise in estradiol. PMID: 18400447
5. The effect of a single nucleotide polymorphism of the follicle stimulating hormone beta chain on bull fertility and semen quality is reported. PMID: 18829190
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亚细胞定位：

Secreted.
蛋白家族：

Glycoprotein hormones subunit beta family
数据库链接：

KEGG: bta:281171

STRING: 9913.ENSBTAP00000014462

UniGene: Bt.406

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.