Bovine fibroblast growth factor 21 (FGF21) ELISA kit

产品详情

产品描述：

牛成纤维细胞生长因子21(FGF21)酶联免疫试剂盒（CSB-EL008627BO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的FGF21含量。FGF21，即成纤维细胞生长因子21，是一种具有多效性的代谢调节剂，对心血管系统具有保护作用，主要通过与FGFR受体的相互作用发挥作用，参与代谢调节，与肥胖和糖尿病等代谢综合征相关。试剂盒检测范围为62.5 pg/mL-4000 pg/mL，适用于兽医科学研究、动物生理学实验及畜牧领域代谢相关分子机制探索，例如在饲料效率优化、代谢综合征模型构建等研究中定量分析FGF21的表达动态本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

FGF21
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

62.5 pg/mL-4000 pg/mL
灵敏度：

15.6 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FGF21 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	88
	Range %	82-93
1:2	Average %	101
	Range %	95-105
1:4	Average %	97
	Range %	92-101
1:8	Average %	88
	Range %	83-96

回收率：

The recovery of bovine FGF21 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	85	81-90
EDTA plasma (n=4)	99	95-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.