Bovine anti-Mullerian hormone (AMH) ELISA kit

Bovine anti-Mullerian hormone (AMH) ELISA kit

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中文名称：

牛缪勒管抑制物质/抗缪勒管激素(AMH)酶联免疫试剂盒
货号：

CSB-E13406B
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO牛缪勒管抑制物质/抗缪勒管激素（AMH）酶联免疫检测试剂盒（货号CSB-E13406B），基于竞争法原理定量检测血清、血浆及组织匀浆样本中的AMH浓度。AMH作为调节生殖系统发育的重要因子，在牛类性别分化、卵泡发育及生殖功能评估中具有关键作用，其表达水平常被用于科研领域评估动物生殖生理状态。该试剂盒检测范围覆盖0.5-200 ng/mL，可精准测定低浓度样本（如幼年个体）至高浓度样本（如性成熟个体）的AMH含量，操作流程通过包被特异性抗体与样本中AMH的竞争性结合反应实现定量分析。适用于畜牧业中繁殖能力研究、卵巢储备功能评估、生殖相关疾病模型构建等科研场景，组织匀浆检测功能还可拓展至生殖器官局部AMH表达分析。试剂盒提供标准品及预包被板条，满足实验室对牛科动物生殖内分泌研究的标准化检测需求，为繁殖生物学、发育生物学等领域的科研工作者提供可靠工具。
别名：

AMH ELISA Kit; Muellerian-inhibiting factor ELISA Kit; Anti-Muellerian hormone ELISA Kit; AMH ELISA Kit; Muellerian-inhibiting substance ELISA Kit; MIS ELISA Kit
缩写：

AMH
Uniprot No.：

P03972
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.5 ng/mL-200 ng/mL
灵敏度：

0.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine AMH in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	90-101
1:2	Average %	98
	Range %	91-105
1:4	Average %	92
	Range %	88-97
1:8	Average %	97
	Range %	93-105

回收率：

The recovery of bovine AMH spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-99
EDTA plasma (n=4)	97	90-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
200	0.134	0.139	0.137
37.5	0.211	0.203	0.207
6.25	0.495	0.485	0.490
1.75	0.968	0.987	0.978
0.5	1.146	1.154	1.150
0	2.226	2.250	2.238

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

This glycoprotein, produced by the Sertoli cells of the testis, causes regression of the Muellerian duct. It is also able to inhibit the growth of tumors derived from tissues of Muellerian duct origin.
基因功能参考文献：
1. Anti-Mullerian hormone inhibits activation and growth of bovine ovarian follicles in vitro and is localized to growing follicles. PMID: 28333275
2. This study showed that antral follicle counts (AFC) and AMH levels were repeatable when determined at an unknown stage of follicular growth and expected day of follicular wave emergence, but repeatability was greater for AMH than AFC. PMID: 28237349
3. The effects of castration and other surgical intervention on the blood levels of anti-Muellerian hormone, inhibin A, gonadotropins, and gonadotropin receptors in bull calves are reported. PMID: 27693012
4. Results from these studies indicate that AMH signaling plays a role in both regulating granulosa cell proliferation and preventing granulosa cells from 5- to 8-mm follicles from undergoing premature differentiation before follicle selection. PMID: 27268296
5. These findings indicate the followings: AMH mRNA levels decrease in both dominant and secondary follicles during follicular deviation; granulosa cells from heathy follicles express more AMH mRNA compared to subordinate follicles undergoing atresia and FSH stimulates AMH and AMHR2 mRNA expression in granulosa cells of co-dominant follicles. PMID: 26815645
6. Measurement of AMH concentration in the plasma of cows can help to predict their capacity for embryo production in response to gonadotrophin treatment. PMID: 22935154
7. In first study to investigate the blood profile and immunohuistochemistry of anti-Mullerian hormone in bovine granulosa-theca cell tumors, the findings indicated that anti-Mullerian hormone is a novel biomarker for granulosa-theca cell tumors in cattle. PMID: 22052009
8. Regulation of anti-Mullerian hormone production in the cow. PMID: 21076084
9. Intrafollicular AMH was not a marker of cystic development in the cow, but low AMH concentrations in cysts were associated with luteinization. PMID: 18448844
10. AMH expression is modulated by androgens in bovine granulosa cells from small follicles. PMID: 19714502
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亚细胞定位：

Secreted.
蛋白家族：

TGF-beta family
数据库链接：

KEGG: bta:280718

STRING: 9913.ENSBTAP00000019912

UniGene: Bt.7050

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.