Bovine Vitamin D-binding protein(GC) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛维生素D结合蛋白(GC)酶联免疫试剂盒（CSB-EL009306BO）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的GC含量。试剂盒检测范围为0.156 μg/mL-10 μg/mL，适用于科研领域中对牛类动物维生素D代谢通路研究、营养状态评估、骨骼发育相关机制探索以及免疫调节功能分析，可为畜牧营养学、兽医学及基础医学研究提供可靠数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

GCVitamin D-binding protein ELISA kit; DBP ELISA kit; VDB ELISA kit; Gc-globulin ELISA kit; Group-specific component ELISA kit
缩写：

GC
Uniprot No.：

Q3MHN5
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.156 μg/mL-10 μg/mL
灵敏度：

0.039 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine GC in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	86
	Range %	82-92
1:200	Average %	93
	Range %	89-97
1:400	Average %	92
	Range %	84-96
1:800	Average %	95
	Range %	90-100

回收率：

The recovery of bovine GC spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	91	85-96
EDTA plasma (n=4)	103	100-106

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Involved in vitamin D transport and storage, scavenging of extracellular G-actin, enhancement of the chemotactic activity of C5 alpha for neutrophils in inflammation and macrophage activation.
基因功能参考文献：
1. Associations between SNPs and the studied traits were strongest for SNPs that were located within and immediately upstream of the group-specific component (GC) gene. Our results suggest GC as the gene that underlies the QTL for clinical mastitis and milk production. PMID: 27760518
亚细胞定位：

Secreted.
蛋白家族：

ALB/AFP/VDB family
数据库链接：

KEGG: bta:530076

STRING: 9913.ENSBTAP00000033386

UniGene: Bt.53584