Bovine Vitamin A (VA) ELISA Kit
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中文名称:牛维生素A(VA)酶联免疫试剂盒
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货号:CSB-E17987B
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规格:96T/48T
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价格:¥3800/¥2500
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其他:
产品详情
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产品描述:牛维生素A(VA)酶联免疫试剂盒(CSB-E17987B)为竞争法ELISA试剂盒,定量检测血清、血浆、组织匀浆样本中的VA含量。试剂盒检测范围为6.25 ng/mL-100 ng/mL,灵敏度为4.672 ng/mL。本试剂盒适用于科研场景中牛类样本的维生素A水平分析,例如探究饲料营养干预对VA吸收的影响、疾病状态下VA代谢变化或组织分布特征等方向本品仅用于科研,不用于临床诊断,产品具体参数及操作步骤详见产品说明书。
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缩写:VA
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种属:Bos taurus (Bovine)
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样本类型:serum, plasma, tissue homogenates
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检测范围:6.25 ng/mL-100 ng/mL
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灵敏度:4.672 ng/mL
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反应时间:1-5h
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样本体积:50-100ul
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检测波长:450 nm
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研究领域:Metabolism
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测定原理:quantitative
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测定方法:Competitive
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精密度:
Intra-assay Precision (Precision within an assay): CV%<8% Three samples of known concentration were tested twenty times on one plate to assess. Inter-assay Precision (Precision between assays): CV%<10% Three samples of known concentration were tested in twenty assays to assess. -
线性度:
To assess the linearity of the assay, samples were spiked with high concentrations of bovine VA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay. Sample Serum(n=4) 1:10 Average % 95 Range % 91-99 1:20 Average % 103 Range % 99-107 1:40 Average % 98 Range % 93-103 1:80 Average % 92 Range % 89-95 -
回收率:
The recovery of bovine VA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section. Sample Type Average % Recovery Range Serum (n=5) 99 93-104 EDTA plasma (n=4) 89 83-95 -
标准曲线:
These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed. ng/ml OD1 OD2 Average 100 0.098 0.095 0.097 50 0.150 0.163 0.157 25 0.263 0.268 0.266 12.5 0.551 0.565 0.558 6.25 1.037 1.065 1.051 0 2.266 2.287 2.277 -
数据处理:
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货期:3-5 working days
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最新研究进展:最新研究表明,VA蛋白在细胞分化、免疫应答和炎症反应等生物学过程中起着重要的作用。
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