Bovine Serum amyloid A,SAA ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

本产品是一款基于竞争性酶联免疫吸附测定原理开发的定量检测试剂盒，专用于牛血清、血浆及组织匀浆样本中淀粉样蛋白A（SAA）的科研分析。SAA作为一种急性期反应蛋白，在机体感染、炎症或组织损伤时显著升高，是评估牛类炎症反应的重要生物标志物。试剂盒检测灵敏度达0.1 μg/mL，线性范围为0.1-20 μg/mL，可精准覆盖牛体内SAA的生理及病理浓度变化。通过预包被特异性抗体的96孔板与样本中SAA及酶标抗原的竞争性结合反应，配合优化的显色体系实现目标蛋白的定量检测。该试剂盒适用于畜牧兽医研究、动物疾病模型构建等领域，可用于探究炎症相关疾病的分子机制、评估疫苗或药物的免疫调节效果，以及监测饲养环境中牛群健康状态，为科研人员提供稳定可靠的实验数据支持。
别名：

SAA1 ELISA Kit; SAA ELISA Kit; Serum amyloid A protein ELISA Kit; SAA) [Cleaved into: Amyloid protein A ELISA Kit; Amyloid fibril protein AA)] ELISA Kit
缩写：

SAA1
Uniprot No.：

P35541
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.1 μg/mL-20 μg/mL
灵敏度：

0.05 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SAA in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-96
1:2	Average %	93
	Range %	89-98
1:4	Average %	95
	Range %	91-98
1:8	Average %	92
	Range %	90-95

回收率：

The recovery of bovine SAA spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	90-99
EDTA plasma (n=4)	96	89-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Major acute phase reactant. Apolipoprotein of the HDL complex.
基因功能参考文献：
1. polymorphisms in the SAA2 gene are associated with milk production traits in Chinese Holstein cows PMID: 26373797
相关疾病：

Reactive, secondary amyloidosis is characterized by the extracellular accumulation in various tissues of the SAA protein. These deposits are highly insoluble and resistant to proteolysis; they disrupt tissue structure and compromise function.
亚细胞定位：

Secreted.
蛋白家族：

SAA family
组织特异性：

Expressed by the liver; secreted in plasma.
数据库链接：

KEGG: bta:506412

STRING: 9913.ENSBTAP00000030310

UniGene: Bt.88760