Bovine Selenoprotein P(SEPP1) ELISA kit

产品详情

产品描述：

牛硒蛋白P（SEPP1）是硒元素在生物体内的重要运输和储存形式，广泛参与抗氧化防御、免疫调节及甲状腺激素代谢等生理过程。本试剂盒（货号：CSB-EL021018BO）基于竞争法原理开发，可定量检测牛血清、血浆及组织匀浆样本中的SEPP1蛋白，检测灵敏度达10 ng/mL，线性范围覆盖10 ng/mL至160 ng/mL，适用于科研领域对硒代谢相关机制的深入研究。试剂盒采用预包被特异性抗体，通过标准品与样本中SEPP1竞争结合标记物实现定量分析，实验步骤标准化且重复性良好，支持多样本类型的高通量检测。该产品可广泛应用于动物营养学研究中的硒元素吸收评估、氧化应激相关疾病模型的生物标志物分析，或环境毒理学中重金属暴露对硒蛋白代谢影响的探索，为畜牧健康管理及基础医学研究提供可靠的数据支持。所有检测结果仅限科学研究使用，操作需严格遵循说明书规范。
别名：

SELENOP ELISA Kit; Selenoprotein P ELISA Kit; SeP ELISA Kit; Selenoprotein P-like protein ELISA Kit
缩写：

SEPP1
Uniprot No.：

P49907
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

10 ng/mL-160 ng/mL
灵敏度：

5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine SEPP1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	89
	Range %	86-102
1:200	Average %	97
	Range %	91-105
1:400	Average %	93
	Range %	88-100
1:800	Average %	91
	Range %	87-96

回收率：

The recovery of bovine SEPP1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	86-99
EDTA plasma (n=4)	93	85-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Constitutes a major selenium pool in the brain and may play an important role in developing and/or modulating the morphology of neurons and/or glial cells.
亚细胞定位：

Secreted.
蛋白家族：

Selenoprotein P family
组织特异性：

Brain and kidney. Most prominently expressed in the cerebellar cortex, hippocampus and olfactory bulb.
数据库链接：

KEGG: bta:282066

UniGene: Bt.64610