Bovine Pregnancy Specific Protein B(PSPB)ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛妊娠特异性蛋白B（PSPB）酶联免疫试剂盒（CSB-E13353B）为竞争法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的PAG1含量。PAG1是重要靶点。它在细胞信号传导等方面发挥作用，与细胞生长、增殖等生理过程相关。研究其机制有助于了解疾病发生发展，可能为肿瘤等疾病的治疗提供新思路，通过调控PAG1有望开发出新型治疗手段。试剂盒检测范围为0.06 ng/mL-24 ng/mL，本产品适用于畜牧科研中妊娠状态监测、胎盘功能评估、生殖内分泌研究及动物繁殖性能分析等场景，其宽泛的样本兼容性可满足血清/血浆直接检测及组织样本经匀浆处理后的定量需求。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Pregnancy-associated glycoprotein 1 ELISA Kit; PAG 1 ELISA Kit; EC 3.4.23.- ELISA Kit; Pregnancy-specific protein B ELISA Kit; PSP-B ELISA Kit
缩写：

PAG1
Uniprot No.：

Q29432
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.06 ng/mL-24 ng/mL
灵敏度：

0.06 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Signal Transduction
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine PSPB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
Range %	90-104
1:2	Average %	94
Range %	88-105
1:4	Average %	90
Range %	85-96
1:8	Average %	97
Range %	89-105

回收率：

The recovery of bovine PSPB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	90	84-94
EDTA plasma (n=4)	97	90-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Appears to be proteolytically inactive.
基因功能参考文献：
1. This study examines expression patterns of the genes SERPINA14, PAG, and PAG2 at the fetal-maternal interface in dairy heifers experimentally infected with N caninum during the second trimester of pregnancy, when most abortions takes place in natural conditions. PMID: 27045629
2. The clinical implications of these findings are that following infection with N. caninum, most cows will experience some level of placental damage and that this injury correlates with foetal fluid PAG levels and pH. PMID: 26936628
3. These findings indicate that IFN-gamma production correlates negatively and the production of antibodies against N. caninum is uncorrelated with plasma pregnancy-associated glycoproteins levels. PMID: 24456132
4. Restraint stress for 2 h during early pregnancy in heifers increased blood cortisol, but it did not affect the concentrations of progesterone and PAG-1 between days 30 to 40 of gestation. PMID: 21872319
亚细胞定位：

Secreted, extracellular space.
蛋白家族：

Peptidase A1 family
组织特异性：

Trophoblast and placental tissue. Produced specifically in the invasive binucleate cells of the placenta. Becomes detectable in maternal serum soon after implantation.
数据库链接：

KEGG: bta:281964

STRING: 9913.ENSBTAP00000025593

UniGene: Bt.15401