Bovine Myoglobin(MB) ELISA kit

产品详情

产品描述：

牛肌红蛋白(MB)酶联免疫试剂盒（CSB-EL013529BO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的MB含量。试剂盒检测范围为15.6 ng/mL-1000 ng/mL，常用于动物肌肉生理研究、运动医学模型构建及肉品加工过程中肌肉品质评估等科研领域。适用于探究牛肌肉疾病机制、运动生理学实验或肌肉相关生物标志物筛选等研究，为畜牧科学、食品科学及基础医学研究提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

MB ELISA Kit; Myoglobin ELISA Kit
缩写：

MB
Uniprot No.：

P02192
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue Homogenates
检测范围：

15.6 ng/mL-1000 ng/mL
灵敏度：

3.9 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine MB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	83-99
1:2	Average %	91
	Range %	89-99
1:4	Average %	95
	Range %	90-100
1:8	Average %	95
	Range %	86-98

回收率：

The recovery of bovine MB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	87-99
EDTA plasma (n=4)	95	90-102

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Serves as a reserve supply of oxygen and facilitates the movement of oxygen within muscles.
基因功能参考文献：
1. These results suggest an important role of genetics in meat color variation for cattle raised under the tropic conditions. PMID: 26946475
2. Glycine at E14 in myoglobin enhances autoxidation and hemin loss rates. PMID: 22681513
蛋白家族：

Globin family
数据库链接：

KEGG: bta:280695

STRING: 9913.ENSBTAP00000007014

UniGene: Bt.107415