Bovine Interleukin 2,IL-2 ELISA Kit

Bovine Interleukin 2,IL-2 ELISA Kit

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中文名称：

牛白介素2(IL-2)酶联免疫试剂盒
货号：

CSB-E09810b
规格：

96T/48T
价格：

￥3800/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

本试剂盒为牛白介素2（IL-2）定量检测专用，采用双抗体夹心ELISA原理，可精准分析血清、血浆及组织匀浆样本中31.25-2000 pg/mL浓度范围的IL-2蛋白。牛IL-2是由活化的T淋巴细胞分泌的关键细胞因子，在免疫调节中发挥核心作用，包括促进T细胞增殖、增强自然杀伤细胞活性及参与免疫耐受形成。试剂盒包含预包被特异性抗体的96孔板（可拆分12×8孔条），配合HRP酶标记检测抗体及TMB显色系统，通过标准品建立标准曲线实现定量分析，操作流程包含4小时37℃一抗孵育和30分钟酶标二抗反应，显色15分钟内通过450nm波长测定吸光度值。适用于科研领域探究牛类免疫机制、疫苗效力评估、畜牧病原感染模型中的细胞免疫应答分析，以及炎症性疾病或免疫抑制相关研究。试剂盒组分包含标准品、检测抗体、终止液等核心试剂，保存于2-8℃条件下有效期达6个月，样本需提前离心或匀浆处理以保障检测稳定性。
别名：

IL2 ELISA kit; IL-2Interleukin-2 ELISA kit; IL-2 ELISA kit; T-cell growth factor ELISA kit; TCGF ELISA kit
缩写：

IL2
Uniprot No.：

P05016
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

31.25 pg/mL-2000 pg/mL
灵敏度：

7.8 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-2 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	85
	Range %	81-90
1:2	Average %	100
	Range %	97-103
1:4	Average %	101
	Range %	98-105
1:8	Average %	94
	Range %	86-98

回收率：

The recovery of bovine IL-2 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	94	89-97
EDTA plasma (n=4)	96	92-98

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
2000	2.151	2.099	2.125	2.040
1000	1.683	1.656	1.670	1.585
500	1.284	1.270	1.277	1.192
250	0.846	0.833	0.840	0.755
125	0.502	0.498	0.500	0.415
62.5	0.367	0.356	0.362	0.277
31.25	0.221	0.213	0.217	0.132
0	0.086	0.084	0.085

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

The relationship between serum cytokine profile and vitamin D in calves with neonatal diarrhea M Caliskan,Cytokine,2023
Association of the MACROD2 rs6110695 A>G polymorphism with an increasing WBC count in a Korean population J Yang,Immunity, inflammation and disease,2022
Enhancing dietary cation-anion difference reshaped the distribution of endotoxin across different biofluids and influenced inflammatory response in dairy cows … Z.Wang,Animal Feed Science and Technology,2020
Alteration in some pro and anti-inflammatory cytokines associated with complete and incomplete gestation cycle of cows Seid Mohammed.et al,Biological Rhythm Research ,2017
Dietary effects of lutein-fortified chlorella on milk components of Holstein cows. Jeon JY.et al,Springerplus.,2016
Kinetics of selected plasma cytokines during innate-adaptive immune response transition in adult cattle infected with the bovine ephemeral fever virus R. Barigye.et al,Veterinary Microbiology,2016

功能：

Produced by T-cells in response to antigenic or mitogenic stimulation, this protein is required for T-cell proliferation and other activities crucial to regulation of the immune response. Can stimulate B-cells, monocytes, lymphokine-activated killer cells, natural killer cells, and glioma cells.
基因功能参考文献：
1. Pretreatment of neonatal PBMC with IL-1beta, TNF-alpha or IFN-gamma promotes mitogenic response to ConA through up-regulating the production of IL-2 and the expression of the mature IL-2 receptor. PMID: 12906107
亚细胞定位：

Secreted.
蛋白家族：

IL-2 family
数据库链接：

KEGG: bta:280822

STRING: 9913.ENSBTAP00000027819

UniGene: Bt.341

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.