Bovine Interleukin 12,IL-12 ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO试剂盒采用双抗体夹心法对牛白介素12（IL-12）进行定量检测，适用于血清、血浆及组织匀浆样本的科研分析。IL-12作为重要的促炎细胞因子，在Th1细胞分化和细胞免疫激活中起关键作用，其表达水平与宿主抗感染免疫应答密切相关。该试剂盒检测灵敏度达到6.25 pg/mL，线性范围覆盖6.25-400 pg/mL浓度梯度，可精准检测不同生物样本中IL-12的动态变化。实验操作采用预包被特异性抗体的96孔板，通过生物素标记检测抗体与链霉亲和素-HRP系统实现信号放大，配套标准品和显色底物完成定量分析。该产品为研究牛类感染性疾病、炎症反应机制及疫苗免疫效果评估提供可靠工具，尤其适用于畜牧科研中免疫调节机制探索、疾病模型建立等基础研究，实验全程约需4.5小时完成，实验方案兼容常规酶标仪检测系统。
别名：

IL12AInterleukin-12 subunit alpha ELISA Kit; IL-12A ELISA Kit; Cytotoxic lymphocyte maturation factor 35 kDa subunit ELISA Kit; CLMF p35 ELISA Kit; IL-12 subunit p35 ELISA Kit
缩写：

IL12A
Uniprot No.：

P54349
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

6.25 pg/mL-400 pg/mL
灵敏度：

1.56 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine IL-12 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	86
	Range %	80-92
1:2	Average %	98
	Range %	91-102
1:4	Average %	94
	Range %	90-98
1:8	Average %	94
	Range %	86-99

回收率：

The recovery of bovine IL-12 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	89	84-92
EDTA plasma (n=4)	97	95-101

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Cytokine that can act as a growth factor for activated T and NK cells, enhance the lytic activity of NK/lymphokine-activated killer cells, and stimulate the production of IFN-gamma by resting PBMC.
基因功能参考文献：
1. The results showed that the expression of TNF-alpha, iNOS, and IL-6 in alveolar macrophages was up-regulated by stimulation with the recombinant Mce4A protein of M. bovis; in contrast, expression of IL-12 was unaffected.[IL-6, IL-12] PMID: 17530193
2. Lipopolysaccharide stimulation up-regulates the secretion of cytokines, including interleukin-12, by bovine polymorphonuclear neutrophil leukocytes. PMID: 17727806
亚细胞定位：

Secreted.
蛋白家族：

IL-6 superfamily
数据库链接：

KEGG: bta:281856

STRING: 9913.ENSBTAP00000020153

UniGene: Bt.524