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  4. Bovine Haptoglobin,Hpt/HP ELISA KIT

Bovine Haptoglobin,Hpt/HP ELISA KIT

文献引用 (3) 说明书下载
  • 中文名称：
    牛结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒
  • 货号：
    CSB-E08585b
  • 规格：
    96T/48T
  • 价格：
    ￥4200/￥3000
  • 其他：
CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

产品详情

  • 产品描述：
    牛结合珠蛋白/触珠蛋白(Hpt/HP)酶联免疫试剂盒（CSB-E08585b）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的HP含量。牛结合珠蛋白（Haptoglobin，Hp）是一种在哺乳动物血清中广泛存在的酸性糖蛋白，主要功能是与游离血红蛋白结合，引导其进入降解代谢程序，防止其对机体的损伤。其亚型与多种疾病如感染、糖尿病、肿瘤等存在相关性，具有作为治疗药物的潜力。牛结合珠蛋白的分离纯化方法包括硫酸铵沉淀、聚乙二醇沉淀等，这些方法有助于大规模纯化结合珠蛋白，为后续的药物开发提供材料。试剂盒检测范围为7.81 ng/mL-2000 ng/mL，该产品适用于畜牧科研中炎症相关疾病的机制研究、疫苗或药物干预效果评估，以及动物健康监测等场景，为牛群疾病模型建立、营养代谢研究提供关键数据支持。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
  • 别名：
    HPHaptoglobin [Cleaved into: Haptoglobin alpha chain; Haptoglobin beta chain] ELISA Kit
  • 缩写：
    HP
  • Uniprot No.：
  • 种属：
    Bos taurus (Bovine)
  • 样本类型：
    serum, plasma
  • 检测范围：
    7.81 ng/mL-2000 ng/mL
  • 灵敏度：
    7.81 ng/mL
  • 反应时间：
    1-5h
  • 样本体积：
    50-100ul
  • 检测波长：
    450 nm
  • 研究领域：
    Cardiovascular
  • 测定原理：
    quantitative
  • 测定方法：
    Competitive
  • 精密度：
    Intra-assay Precision (Precision within an assay): CV%<8%      
    Three samples of known concentration were tested twenty times on one plate to assess.  
    Inter-assay Precision (Precision between assays): CV%<10%      
    Three samples of known concentration were tested in twenty assays to assess.    
                 
  • 线性度：
    To assess the linearity of the assay, samples were spiked with high concentrations of bovine Hpt in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
      Sample Serum(n=4)  
    1:1000 Average % 84  
    Range % 80-88  
    1:2000 Average % 90  
    Range % 85-94  
    1:4000 Average % 103  
    Range % 99-112  
    1:8000 Average % 98  
    Range % 94-102  
  • 回收率：
    The recovery of bovine Hpt spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.
    Sample Type Average % Recovery Range  
    Serum (n=5) 95 90-99  
    EDTA plasma (n=4) 92 88-96  
                 
                 
  • 标准曲线：
    These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.
    ng/ml OD1 OD2 Average    
    2000 0.243 0.252 0.248    
    500 0.412 0.436 0.424    
    120 0.793 0.754 0.774    
    31.25 1.214 1.239 1.227    
    7.81 1.795 1.724 1.760    
    0 2.405 2.359 2.382    
  • 数据处理：
    ELISA操作视频及标曲绘制软件下载
  • 货期：
    3-5 working days

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HP Proteins

相关问答

■ 常见问题解答
Q:

a. Is the capture antibody a monoclonal or polyclonal?
b. What is the host of the capture antibody?
c. Is the detection antibody a monoclonal or polyclonal?
d. What is the host of the detection antibody?
e. What’s is that standard? A recombinant protein or something else? If a recombinant protein, what is the host?
f. If recombinant protein, is it full length or partial?
g. What is the species of the standard– human or something else?

A:
Thanks for your inquiry.
The antibody used in the kit is rabbit polyclonal antibody.
The standard is purified crude extract of native samples.
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靶点详情

  • 功能：
    As a result of hemolysis, hemoglobin is found to accumulate in the kidney and is secreted in the urine. Haptoglobin captures, and combines with free plasma hemoglobin to allow hepatic recycling of heme iron and to prevent kidney damage. Haptoglobin also acts as an antioxidant, has antibacterial activity and plays a role in modulating many aspects of the acute phase response. Hemoglobin/haptoglobin complexes are rapidly cleared by the macrophage CD163 scavenger receptor expressed on the surface of liver Kupfer cells through an endocytic lysosomal degradation pathway.
  • 基因功能参考文献：
    1. These results indicate that it is possible to monitor the course of pyometra in cows based on the evaluation of the concentration of cytokines and Hp in the serum and uterine washings. PMID: 25704413
    2. These data demonstrate that serum neutrophil haptoglobin-MMP 9 complexes appear sooner and decline more rapidly than other acute phase proteins. PMID: 25358728
    3. An increase in serum amyloid A or haptoglobin in dairy cows with abomasal displacementis not specific for inflammation or bacterial infection; increased levels of either may indicate the presence of hepatic lipidosis. PMID: 20391639
    4. Haptoglobin concentration may be a useful indicator for cows with postpartum reproductive disorders. PMID: 14960809
    5. Presence in the milk of the acute phase proteins (APP), haptoglobin (Hp) and serum amyloid A to see the relationships in cow composite and bulk tank milk samples. PMID: 17227596
    6. Bovine granulocytes, isolated from peripheral blood of healthy cattle, contain abundant amounts of haptoglobin within the granules. PMID: 17681384
    7. This study suggests that neutrophils and epithelial cells may play an essential role in elevating milk haptoglobin (Hp) in addition to previous suggestions that Hp may be derived from mammary tissues and circulation. PMID: 19094922

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  • 亚细胞定位：
    Secreted, extracellular space.
  • 蛋白家族：
    Peptidase S1 family
  • 组织特异性：
    Expressed by the liver and secreted in plasma.
  • 数据库链接：

    KEGG: bta:280692

    STRING: 9913.ENSBTAP00000008335

    UniGene: Bt.12553

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