Bovine Fibronectin,FN ELISA Kit

中文名称：

牛纤连蛋白(FN)酶联免疫试剂盒
货号：

CSB-E13781B
规格：

96T/48T
价格：

￥3600/￥2500
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

CUSABIO牛纤连蛋白（Fibronectin, FN）酶联免疫检测试剂盒（货号：CSB-E13781B），采用双抗体夹心法定量检测样本中FN的浓度。纤连蛋白是一种广泛存在于细胞外基质和体液中的高分子量糖蛋白，参与细胞粘附、迁移及组织修复等生物学过程，在胚胎发育、伤口愈合和炎症调控中发挥重要作用。本试剂盒适用于检测血清、血浆、细胞培养上清液、组织匀浆及细胞裂解液等多种样本类型，检测灵敏度达0.137μg/mL，线性范围为0.137-100μg/mL，可精准测定低丰度样本中的FN含量。该试剂盒通过预包被高特异性捕获抗体与检测抗体协同作用，结合酶标系统显色反应实现定量分析，操作便捷且重复性良好。科研人员可将其应用于细胞外基质功能研究、细胞粘附机制探索、组织再生模型构建或炎症相关疾病模型的分子机制分析，例如评估体外培养细胞分泌FN的水平，或分析特定病理状态下组织微环境中FN的动态变化。本产品仅限科学研究使用。
别名：

FN1Fibronectin ELISA Kit; FN) [Cleaved into: Anastellin] ELISA Kit
缩写：

FN1
Uniprot No.：

P07589
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates, cell lysates
检测范围：

0.137 μg/mL-100 μg/mL
灵敏度：

0.137 μg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine FN in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:5	Average %	92
	Range %	85-102
1:10	Average %	97
	Range %	91-110
1:20	Average %	89
	Range %	83-96
1:40	Average %	95
	Range %	84-100

回收率：

The recovery of bovine FN spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	96	88-105
EDTA plasma (n=4)	97	92-105

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

µg/ml	OD1	OD2	Average	Corrected
100	1.839	1.803	1.821	1.677
33	1.657	1.649	1.653	1.509
11	1.451	1.435	1.443	1.299
3.7	0.958	0.992	0.975	0.831
1.2	0.643	0.667	0.655	0.511
0.4	0.372	0.354	0.363	0.219
0.13	0.251	0.243	0.247	0.103
0	0.145	0.142	0.144

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Fibronectins bind cell surfaces and various compounds including collagen, fibrin, heparin, DNA, and actin. Fibronectins are involved in cell adhesion, cell motility, opsonization, wound healing, and maintenance of cell shape. Involved in osteoblast compaction through the fibronectin fibrillogenesis cell-mediated matrix assembly process, essential for osteoblast mineralization. Participates in the regulation of type I collagen deposition by osteoblasts.; Binds fibronectin and induces fibril formation. This fibronectin polymer, named superfibronectin, exhibits enhanced adhesive properties. Both anastellin and superfibronectin inhibit tumor growth, angiogenesis and metastasis. Anastellin activates p38 MAPK and inhibits lysophospholipid signaling.
亚细胞定位：

Secreted, extracellular space, extracellular matrix.
组织特异性：

Plasma FN (soluble dimeric form) is secreted by hepatocytes. Cellular FN (dimeric or cross-linked multimeric forms), made by fibroblasts, epithelial and other cell types, is deposited as fibrils in the extracellular matrix.
数据库链接：

STRING: 9913.ENSBTAP00000010925

UniGene: Bt.23418

Bovine Fibronectin,FN ELISA Kit

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