Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

中文名称：

牛脱氧核糖核酸酶1(DNASE1)酶联免疫试剂盒
货号：

CSB-EL007049BO
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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CUSABIO牛脱氧核糖核酸酶1（DNASE1）酶联免疫检测试剂盒（货号CSB-EL007049BO），采用双抗体夹心法定量检测样本中DNASE1的浓度。DNASE1是一种参与DNA降解和细胞凋亡过程的关键酶，在维持基因组稳定性及调控免疫反应中发挥重要作用。该试剂盒检测灵敏度范围为12.5至800 pg/mL，适用于牛血清、血浆及组织匀浆样本的检测，实验操作基于预包被特异性抗体的微孔板，通过标准品梯度稀释建立定量曲线，结合酶标二抗显色反应实现目标蛋白的精准定量。该试剂盒具有高特异性和重复性，可为科研领域研究牛DNASE1在细胞凋亡机制、炎症反应或免疫调节中的功能提供可靠工具，尤其适用于畜牧病理学、动物免疫学研究中对相关生物标志物的定量分析需求。
别名：

DNASE1 ELISA Kit; DNL1Deoxyribonuclease-1 ELISA Kit; EC 3.1.21.1 ELISA Kit; Deoxyribonuclease I ELISA Kit; DNase I ELISA Kit
缩写：

DNASE1
Uniprot No.：

P00639
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

12.5 pg/mL-800 pg/mL
灵敏度：

3.1 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Epigenetics and Nuclear Signaling
测定原理：

quantitative
测定方法：

Sandwich

精密度：

Intra-assay Precision (Precision within an assay): CV%<8%
Three samples of known concentration were tested twenty times on one plate to assess.
Inter-assay Precision (Precision between assays): CV%<10%
Three samples of known concentration were tested in twenty assays to assess.

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine DNASE1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:20	Average %	85
	Range %	80-89
1:40	Average %	94
	Range %	90-98
1:80	Average %	103
	Range %	99-109
1:160	Average %	97
	Range %	92-101

回收率：

The recovery of bovine DNASE1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	88	84-92
EDTA plasma (n=4)	93	89-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

pg/ml	OD1	OD2	Average	Corrected
800	2.399	2.416	2.408	2.273
400	2.213	2.195	2.204	2.069
200	1.813	1.741	1.777	1.642
100	1.204	1.227	1.216	1.081
50	0.683	0.713	0.698	0.563
25	0.400	0.392	0.396	0.261
12.5	0.292	0.284	0.288	0.153
0	0.131	0.138	0.135

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

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功能：

Serum endocuclease secreted into body fluids by a wide variety of exocrine and endocrine organs. Expressed by non-hematopoietic tissues and preferentially cleaves protein-free DNA. Among other functions, seems to be involved in cell death by apoptosis. Binds specifically to G-actin and blocks actin polymerization. Together with DNASE1L3, plays a key role in degrading neutrophil extracellular traps (NETs). NETs are mainly composed of DNA fibers and are released by neutrophils to bind pathogens during inflammation. Degradation of intravascular NETs by DNASE1 and DNASE1L3 is required to prevent formation of clots that obstruct blood vessels and cause organ damage following inflammation.
基因功能参考文献：
1. ascorbic acid has the ability to inhibit DNase I, one of the main endonucleases involved in DNA fragmentation during apoptosis. PMID: 29551685
2. the practical capability of this assay system was successfully demonstrated by its use to determine DNase I activity in bovine urine. PMID: 27570054
3. Specificity in DNA-DNase I interaction is mediated by DNA flexibility, which influences the induced-fit transitions required to form productive complexes. PMID: 19850052
4. results indicated that the backbone hydrogen bonding in the antiparallel beta-sheet formed by its N- and C-termini, rather than the side-chain interactions, is crucial for the correct protein folding PMID: 15311926
亚细胞定位：

Secreted. Zymogen granule. Nucleus envelope.
蛋白家族：

DNase I family
数据库链接：

KEGG: bta:282217

STRING: 9913.ENSBTAP00000054758

UniGene: Bt.12952

Bovine Deoxyribonuclease-1(DNASE1) ELISA kit

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