Bovine Caspase 7(Casp-7) ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛胱天蛋白酶7(Casp-7)酶联免疫试剂盒（CSB-E17315B）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的Casp7含量。Casp7（Caspase-7）是一种参与细胞凋亡过程的半胱氨酸型内肽酶，属于caspase家族。它在细胞凋亡的执行阶段发挥关键作用，通过切割下游底物如PARP来促进程序性细胞死亡。Casp7的表达和活性与多种疾病如癌症、阿尔茨海默病等密切相关。试剂盒检测范围为0.625 ng/mL-40 ng/mL，该产品可用于科研领域细胞凋亡机制研究、药物或毒素诱导的凋亡效应评估、免疫相关疾病模型分析等场景，例如探究病原感染、肿瘤治疗或神经系统疾病中的Casp - 7表达变化。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
缩写：

Casp7
Uniprot No.：

F1MD58
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

0.625 ng/mL-40 ng/mL
灵敏度：

0.156 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cell Biology
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine Casp-7 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	95
	Range %	85-101
1:2	Average %	95
	Range %	88-102
1:4	Average %	89
	Range %	83-100
1:8	Average %	93
	Range %	89-106

回收率：

The recovery of bovine Casp-7 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	97	90-112
EDTA plasma (n=4)	93	84-99

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.