Bovine C-reactive protein,CRP ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

CUSABIO牛C反应蛋白（CRP）酶联免疫检测试剂盒（货号：CSB-E08577b），基于竞争法的定量检测技术，可精准检测牛血清或血浆样本中CRP的浓度，检测范围为1.25-80 ng/mL。C反应蛋白作为重要的急性期反应蛋白，在炎症、感染或组织损伤时显著升高，是评估动物免疫状态及炎症进程的关键生物标志物。CUSABIO试剂盒采用高特异性抗体，通过优化的预包被板及显色系统，可在3小时内完成样本检测，灵敏度达1.25 ng/mL，适用于低丰度样本分析。其操作流程兼容常规实验室设备，适用于兽医研究、畜牧业动物健康监测及炎症相关分子机制探索等科研领域，例如通过动态监测CRP水平评估牛群感染性疾病的发展趋势或疫苗免疫效果。试剂盒提供标准品和实验所需全套组分，支持重复检测，为科研人员提供稳定可靠的数据支持。
缩写：

CRP
Uniprot No.：

C4T8B4
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

1.25 ng/mL-80 ng/mL
灵敏度：

1.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Immunology
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine CRP in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	91
	Range %	86-98
1:200	Average %	97
	Range %	92-102
1:400	Average %	85
	Range %	81-88
1:800	Average %	89
	Range %	83-96

回收率：

The recovery of bovine CRP spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	92	87-98
EDTA plasma (n=4)	97	92-103

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.