Bovine Beta-Hydroxybutyric Acid,β-OHB ELISA Kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<15%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<15%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛β羟丁酸(βOHB)酶联免疫试剂盒（CSB-E10056b）为竞争法ELISA试剂盒，定量检测血清、血浆样本中的β-OHB含量。β-OHB即β-羟基丁酸，是酮体的主要成分之一。它在人体代谢中发挥着重要作用，可作为能量底物供能。研究发现其能通过调节多种信号通路，参与细胞代谢、炎症反应、氧化应激等过程，在神经保护、代谢性疾病等方面研究较多。试剂盒检测范围为50 nmol/mL-800 nmol/mL，适用于畜牧科研领域，例如探究牛群能量代谢特征、营养调控对酮体生成的影响，或作为代谢综合征模型的生物标志物检测工具。实验操作兼容常规实验室设备，为动物营养学、兽医学研究提供高效、稳定的检测方案，数据结果仅用于科学研究，不涉及其他用途。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

beta-Hydroxybutyric acid, beta-OHB
缩写：

β-OHB
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma
检测范围：

50 nmol/mL-800 nmol/mL
灵敏度：

9.5 nmol/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Others
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine β-OHB in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	90
	Range %	88-95
1:2	Average %	96
	Range %	91-103
1:4	Average %	98
	Range %	92-104
1:8	Average %	95
	Range %	89-100

回收率：

The recovery of bovine β-OHB spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	98	90-103
EDTA plasma (n=4)	95	90-100

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.