Bovine Apolipoprotein A-I(APOA1) ELISA kit

Bovine Apolipoprotein A-I(APOA1) ELISA kit

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中文名称：

牛载脂蛋白A1(APOA1)酶联免疫试剂盒
货号：

CSB-EL001913BO
规格：

96T/48T
价格：

￥4200/￥3000
其他：

CUSABIO Wins 2024 Bioz Rapid Star™ Award in the ELISA category

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产品描述：

牛载脂蛋白A1（APOA1）酶联免疫试剂盒（货号：CSB-EL001913BO）是一款基于竞争法原理的定量检测工具，专为分析牛血清、血浆及组织匀浆样本中的APOA1含量而开发。APOA1作为高密度脂蛋白（HDL）的核心组成蛋白，在胆固醇逆向转运和脂质代谢调控中发挥关键作用，其表达水平与心血管健康及代谢性疾病研究密切相关。CUSABIO试剂盒采用高特异性抗体，检测灵敏度达78 ng/mL，线性检测范围为78-5000 ng/mL，可精准量化不同浓度梯度的样本。试剂盒适用于探索牛脂质代谢相关疾病机制、评估营养干预对脂蛋白合成的影响，或作为动物模型中动脉粥样硬化研究的工具，为畜牧养殖健康管理、动物疾病模型构建及基础医学研究提供可靠数据支持。操作流程严格遵循标准化实验方案，兼容常规实验室设备，确保实验结果的高重复性和稳定性。
别名：

APOA1Apolipoprotein A-I ELISA kit; Apo-AI ELISA kit; ApoA-I ELISA kit; Apolipoprotein A1) [Cleaved into: Proapolipoprotein A-I ELISA kit; ProapoA-I); Truncated apolipoprotein A-I] ELISA kit
缩写：

APOA1
Uniprot No.：

P15497
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

78 ng/mL-5000 ng/mL
灵敏度：

19.5 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Cardiovascular
测定原理：

quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine APOA1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1000	Average %	90
	Range %	87-94
1:2000	Average %	96
	Range %	91-100
1:4000	Average %	93
	Range %	87-97
1:8000	Average %	100
	Range %	96-103

回收率：

The recovery of bovine APOA1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	84	82-87
EDTA plasma (n=4)	89	85-96

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

ng/ml	OD1	OD2	Average
5000	0.140	0.143	0.142
2500	0.285	0.291	0.288
1250	0.482	0.503	0.493
625	0.814	0.821	0.818
312	1.253	1.268	1.261
156	1.695	1.709	1.702
78	2.040	2.134	2.087
0	2.436	2.458	2.447

数据处理：

ELISA操作视频及标曲绘制软件下载
货期：

3-5 working days

功能：

Participates in the reverse transport of cholesterol from tissues to the liver for excretion by promoting cholesterol efflux from tissues and by acting as a cofactor for the lecithin cholesterol acyltransferase (LCAT). As part of the SPAP complex, activates spermatozoa motility.
基因功能参考文献：
1. the model of a two-step process for the transendothelial transport of apoA-I in which apoA-I is initially lipidated by ABCA1 and then further processed by ABCA1-independent mechanisms. PMID: 21209084
亚细胞定位：

Secreted.
蛋白家族：

Apolipoprotein A1/A4/E family
组织特异性：

Major protein of plasma HDL, also found in chylomicrons.
数据库链接：

KEGG: bta:281631

STRING: 9913.ENSBTAP00000002914

UniGene: Bt.49157

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.