Bovine Alpha-2-HS-glycoprotein(AHSG) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

牛α2-HS糖蛋白(AHSG)酶联免疫试剂盒（CSB-EL001485BO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织匀浆样本中的AHSG含量。AHSG（α2-HS糖蛋白）是一种由肝脏分泌的血浆糖蛋白，与多种疾病如糖尿病、心血管疾病和结石等密切相关。其具有高度的遗传多态性，其基因多态性与糖尿病、血管钙化等疾病的易感性有关。AHSG在细胞过程中发挥多种作用，如促进内吞作用、调节骨的矿物质相、影响骨稳态等。试剂盒检测范围为125 ng/mL-8000 ng/mL，本产品可为畜牧业中牛群健康研究、营养代谢调控实验提供数据支持，例如骨骼发育研究、炎症相关疾病模型构建或免疫系统功能探索等科研方向，使用者可通过检测血清/血浆样本评估生理状态，或分析肝、肾等组织匀浆中的AHSG表达水平。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

AHSG ELISA Kit; FETUAAlpha-2-HS-glycoprotein ELISA Kit; Asialofetuin ELISA Kit; Fetuin-A ELISA Kit
缩写：

AHSG
Uniprot No.：

P12763
种属：

Bos taurus (Bovine)
样本类型：

serum, plasma, tissue homogenates
检测范围：

125 ng/mL-8000 ng/mL
灵敏度：

31.25 ng/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Metabolism
测定原理：

Quantitative
测定方法：

Competitive

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of bovine AHSG in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:100	Average %	96
	Range %	92-98
1:200	Average %	97
	Range %	93-102
1:400	Average %	102
	Range %	98-106
1:800	Average %	88
	Range %	82-96

回收率：

The recovery of bovine AHSG spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	90-99
EDTA plasma (n=4)	104	99-108

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Promotes endocytosis, possesses opsonic properties and influences the mineral phase of bone. Suggested to have lymphocyte stimulating properties, lipid binding capability and to bind thyroid hormone.
基因功能参考文献：
1. two glycoproteins, AHSG and HPX, represent a pivotal glycoprotein of the cytoprotective activity for WN1316, showing a concrete evidence for the novel glycan-independent function of serum glycoproteins in neuroprotective drug efficacy. PMID: 29016670
2. Site-specific analysis of the O-glycosylation of bovine fetuin by electron-transfer dissociation mass spectrometry. PMID: 24907489
3. These experiments revealed a second mechanism of long-term mineral ion stabilization by the fetuin-A that is complementary to the formation of calciprotein particles. PMID: 21156141
亚细胞定位：

Secreted.
蛋白家族：

Fetuin family
组织特异性：

Liver and bone.
数据库链接：

KEGG: bta:280988

STRING: 9913.ENSBTAP00000000673

UniGene: Bt.23250