Y10A Antibody

^{Rare Species}

产品详情

产品名称：

Rabbit anti-Enterobacteria phage T4 (Bacteriophage T4) Y10A Polyclonal antibody
Uniprot No.：

P32277
基因名：

Y10A
别名：

RNA ligase 2 (Rnl2) (EC 6.5.1.3) Y10A 24.1
宿主：

Rabbit
反应种属：

Enterobacteria phage T4 (Bacteriophage T4)
免疫原：

Recombinant Enterobacteria phage T4 (Bacteriophage T4) Y10A protein (1-249aa)
免疫原种属：

Enterobacteria phage T4 (Bacteriophage T4)
标记方式：

Non-conjugated
克隆类型：

Polyclonal
抗体亚型：

IgG
纯化方式：

>95%, Protein G purified
浓度：

It differs from different batches. Please contact us to confirm it.
保存缓冲液：

Preservative: 0.03% Proclin 300
Constituents: 50% Glycerol, 0.01M PBS, pH 7.4
产品提供形式：

Liquid
应用范围：

ELISA, WB
推荐稀释比：

Application Recommended Dilution

WB 1:500-1:5000
Protocols：

ELISA Protocol
Western Blotting (WB) Protocol
储存条件：

Upon receipt, store at -20°C or -80°C. Avoid repeated freeze.
货期：

Basically, we can dispatch the products out in 1-3 working days after receiving your orders. Delivery time maybe differs from different purchasing way or location, please kindly consult your local distributors for specific delivery time.
用途：

For Research Use Only. Not for use in diagnostic or therapeutic procedures.

功能：

Repairs 3'-OH/5'-PO4 nicks in duplex RNA or RNA:DNA hybrid in which the broken 3'-OH strand is RNA. The nick ligation reaction entails three nucleotidyl transfer steps. In the first step, the RNA ligase reacts with ATP in the absence of nucleic acid to form a covalent ligase-AMP intermediate and release pyrophosphate. In step 2, the ligase-AMP binds to the nicked duplex nucleic acid and transfers the adenylate to the 5'-PO4 terminus to form an adenylylated nicked intermediate. In step 3, the RNA ligase directs the attack of the nick 3'-OH on the 5'-phosphoanhydride linkage, resulting in a repaired 3' - 5' phosphodiester and release of AMP.
基因功能参考文献：
1. To unravel the biochemical and functional relationship between Rnl1, Rnl2, and Dnl, a systematic analysis of their substrate specificity was performed using recombinant proteins PMID: 16671895
2. Results report crystal structures for T4 RNA ligase 2 (Rnl2) at discrete steps along the RNA repair pathway. PMID: 17018278
蛋白家族：

RNA ligase 2 family
数据库链接：

KEGG: vg:1258563