Mouse Chordin-like protein 1(CHRDL1) ELISA kit

产品详情

Intra-assay Precision (Precision within an assay): CV%<8%

Three samples of known concentration were tested twenty times on one plate to assess.

Inter-assay Precision (Precision between assays): CV%<10%

Three samples of known concentration were tested in twenty assays to assess.

产品描述：

小鼠腱蛋白样蛋白1(CHRDL1)酶联免疫试剂盒（CSB-EL005378MO）为双抗夹心法ELISA试剂盒，定量检测血清、血浆、组织培养上清液、组织匀浆样本中的CHRDL1含量。小鼠腱蛋白样蛋白1（CHRDL1）是一种重要的分泌性发育介导蛋白，在多种组织中表达，具有调节细胞命运和促进神经元分化的功能。其研究机制涉及与BMP4结合作为拮抗剂，阻断受体相互作用，并可能参与多种生理和病理过程，如神经发育和癌症预后。试剂盒检测范围为15.6 pg/mL-1000 pg/mL，本试剂盒适用于体外研究CHRDL1在神经生物学、骨骼发育或肿瘤模型中的表达水平变化。为探索CHRDL1的分子功能及调控机制提供可靠工具。本品仅用于科研，不用于临床诊断，产品具体参数及操作步骤详见产品说明书。
别名：

Chrdl1 ELISA kit; Ng1 ELISA kit; Nrln1Chordin-like protein 1 ELISA kit; Neuralin-1 ELISA kit; Neurogenesin-1 ELISA kit; Ventroptin ELISA kit
缩写：

CHRDL1
Uniprot No.：

Q920C1
种属：

Mus musculus (Mouse)
样本类型：

serum, plasma, cell culture supernates, tissue homogenates
检测范围：

15.6 pg/mL-1000 pg/mL
灵敏度：

1.95 pg/mL
反应时间：

1-5h
样本体积：

50-100ul
检测波长：

450 nm
研究领域：

Neuroscience
测定原理：

quantitative
测定方法：

Sandwich

精密度：

线性度：

To assess the linearity of the assay, samples were spiked with high concentrations of mouse CHRDL1 in various matrices and diluted with the Sample Diluent to produce samples with values within the dynamic range of the assay.
	Sample	Serum(n=4)
1:1	Average %	92
	Range %	88-95
1:2	Average %	101
	Range %	97-106
1:4	Average %	98
	Range %	92-103
1:8	Average %	86
	Range %	82-89

回收率：

The recovery of mouse CHRDL1 spiked to levels throughout the range of the assay in various matrices was evaluated. Samples were diluted prior to assay as directed in the Sample Preparation section.

Sample Type	Average % Recovery	Range
Serum (n=5)	95	89-102
EDTA plasma (n=4)	89	85-95

标准曲线：

These standard curves are provided for demonstration only. A standard curve should be generated for each set of samples assayed.

功能：

Seems to antagonize the function of BMP4 by binding to it and preventing its interaction with receptors. Alters the fate commitment of neural stem cells from gliogenesis to neurogenesis. Contributes to neuronal differentiation of neural stem cells in the brain by preventing the adoption of a glial fate. May play a crucial role in dorsoventral axis formation. Antagonizes the function of BMP7 and may thus play an important role in the embryonic bone formation. Shows no inhibitory effect on the inducing activity of BMP2. Plays a role during anterior segment eye development.
基因功能参考文献：
1. CHRDL-1 is a new regulatory target of miR-134 during cortical neuronal differentiation; this is a novel role for miR-134 in the modulation of cortical development. PMID: 21228099
2. Reperfusion injury of proximal kidney tubules causes a reduction of Chrdl1 expression. PMID: 19357253
亚细胞定位：

Secreted.
组织特异性：

Expressed in heart, brain, lung, liver, kidney and testis.
数据库链接：

KEGG: mmu:83453

STRING: 10090.ENSMUSP00000056193

UniGene: Mm.157697